Resume

Chenhui Zhang

E-mail : 12032627@mail.sustech.edu.cn

Education

Southern University of Science and Technology

• Master of Science, Biology
• Sept. 2020 – July 2023
• Supervisor: Yingxia Liu(Professor) and Liang Yang(Professor)

Lanzhou University

• Bachelor of Science, Biology
• Sept. 2016 – July 2020

Primary fields of research

• Cryo-Electron Microscopy
• Pocket prediction and Molecular docking
• Molecular Dynamics Simulation

Publications

• Cryo-EM && Single Particle Analysis:

  1.Yang, Yang, et al. “A novel nanobody broadly neutralizes SARS‐CoV‐2 via induction of spike trimer dimers conformation.” Exploration. 2023. (co-first author)

• Molecular Dynamics Simulation:

  2.Ye, Guoguo, et al. “A peptide derived from SARS‐CoV‐2 nucleocapsid protein with broad‐spectrum anti‐coronavirus activity.” Journal of Medical Virology 96.4 (2024): e29492. (second author)

  3.Sun, Xiao-Yu, et al. “Superoxide dismutase A (SodA) is a c-di-GMP effector protein governing oxidative stress tolerance in Stenotrophomonas maltophilia.” Microbiological Research 278 (2024): 127535. (third author)

• Data Analysis with Python:

  4.Zou, Rongrong, et al. “Antiviral efficacy and safety of molnupiravir against omicron variant infection: a randomized controlled clinical trial.” Frontiers in pharmacology 13 (2022): 939573. (co-author)

Skills & Interests

• Language: Mandarin , English

• Experimental skills: Plasmid Construction,Protein Expression ,Protein purification, Western Blot, Negative-Staining Technology,Sample Preparation for Cryo-EM

• Computer Technology:

  1.Single Particle Analysis: Cryosparc,Relion,Gautomatch,Pymol,AlphaFold2,Chimerax;

  2.Molecular Dynamics Simulation: Gromacs,Charmm-gui,vmd;

  3.Pocket prediction and docking: Autodock vina,ADT,Swiss-modle,ROSETTA, P2RANK,Autosite ,Fpocket ,Openbabel;

  4.Software development: Python ,Java,Anaconda, Sql, Hadoop, HTML, Css, Linux command and Shell;

  5.Others: Markdown,Latex.

• Interests: Cartoon（Family guy，Rick and Morty）

Project Experiences

1. Structural Biology Study of the T6SS-Secreted Esterase Effector Proteins in Pseudomonas aeruginosa [Project member (Master)]

• Expression and purification of the core subunit VgrG2a of Pseudomonas aeruginosa H2-T6SS;
• Optimization of negative staining for the VgrG1a & PA0260-PA0259 complex;
• Sample preparation and Cryo-EM data collection for the VgrG1a & PA0260-PA0259 complex.

2.The structure analysis of the IBT-CoV144-Spike complex [Project main member (Master) ]

• Sample Preparation with FEI Vitrobot Mark IV;
• Using serialEM software collected Cryo-EM data;
• Single particle analysis by Cryosparc and Relion.

3.Building a Molecular Docking and Molecular Dynamics Simulation Platform [Project main member (Research Assistant) ]

• Installing Ubuntu 22.04.4 LTS on a Workstation;
• Compiling and Installing GROMACS and Rosetta from Source code;
• Through the established molecular dynamics simulation platform, the properties of various protein complexes were explored, such as the c-di-GMP and SodA complex, peptides and the SARS-CoV-2 N protein, and the spike protein and nanobody complex.

Awards &Honors

• Excellent Graduate with a Postgraduate Degree(2023)
• President of the Reading Association, Lanzhou University, 2017-2018